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| Rostelato-Ferreira,S; Rodrigues-Simioni,L; Oshima-Franco,Y. |
| The crude venom of Bothrops jararacussu (Bjssu) is known to induce muscular paralysis in vitro. Many studies have shown that various substances, including heparin, neutralize the damage caused by snake venom. In the present study, the ability of heparin (Hep) and commercial bothropic antivenom (CBA) to neutralize neuromuscular effects of Bjssu venom, at different time-points, was analyzed. Mouse phrenic nerve-diaphragm preparation was used through a conventional myographic technique, following five different protocols: Group 1 was incubated with Bjssu (40 µg/mL) without any other treatment; Groups 2 and 3 were pretreated with heparin (1 µL/mL) and CBA (120 µL/mL), respectively, for 15 minutes before venom addition; Group 4 after 50% neuromuscular blockade... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops jararacussu; Heparin; Neutralization; Nerve-muscle preparation. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100005 |
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| Della Torre,A; Albuquerque,LBL; Farrapo,NM; Oshima-Franco,Y; Santos,MG; Tavares,RVS; Rodas,ACD; Dal Belo,CA; Cardoso,CRP; Varanda,EA; Groppo,FC; Lopes,PS. |
| Plathymenia reticulata Benth has an anti-inflammatory effect and is capable of neutralizing the neuromuscular blockade induced by Bothrops jararacussu or Crotalus durissus terrificus venoms, probably by precipitating venom proteins (an effect caused by plant tannins). The present study aimed to evaluate the mutagenic activity of P. reticulata by using the Salmonella mutagenicity assay (Ames test) and the micronucleus test in CHO-K1 cells. P. reticulata extract concentrations of 2.84, 5.68, 11.37, and 19.90 mg/plate were assayed by the Ames test using TA97a, TA98, TA100 and TA102 bacterial strains, with (+S9) and without (-S9) metabolic activation. Concentrations of 5, 1.6 and 0.5 μg/mL of P. reticulata extract were used for the micronucleus test. P.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ames test; Micronucleus test; Mutagenicity; Plant extract; Plathymenia reticulata. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200010 |
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